normal human cervical epithelial cell hucec lines  (ATCC)

Journal: Chinese Medical Journal

Article Title: LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells

doi: 10.1097/CM9.0000000000001665

Figure Lengend Snippet: Level changes of AFAP1-AS1 and stemness in CD44v6(+) CC cells. (A) Volcano plots showing expression profiles of lncRNAs. (B) qRT-PCR detected AFAP1-AS1 levels, ∗ P < 0.05; (C) Western blotting detected CD44v6 levels. ∗ P < 0.05. (D) CD44v6(+)/(−) cells sorting by FCM. (E) AFAP1-AS1 levels were increased in CD44v6(+) cells, ∗ P < 0.05; (F) and (G) Representative images of spheres for CC cells and number of spheres (100×). (H–J) qRT-PCR results showed that OCT4, OPN, CD133 mRNA levels were increased in CD44v6(+) cells. ∗ P < 0.05. (K–N) Western blotting results showed that OCT4, OPN, CD133 protein levels were increased in CD44v6(+) cells, ∗ P < 0.05. AFAP1-AS1: Actin filament-associated protein 1 antisense RNA 1; CD133: Cluster of differentiation 133; CC: Cervical cancer; CD44v6: Hyaluronic acid receptor cluster of differentiation 44 variant exon 6; HUCEC: Human cervical epithelial cell line; LncRNA: Long non-coding RNA; OCT4: Octamer-binding transcription factor 4; OPN: Osteopontin; qRT-PCR: Quantitative real-time PCR.

Article Snippet: We obtained SiHa and Hela, the human CC cells, and normal human cervical epithelial cell line (HUCEC) from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Variant Assay, Binding Assay, Real-time Polymerase Chain Reaction

Journal: Cancer Cell International

Article Title: HAND2-AS1 inhibits invasion and metastasis of cervical cancer cells via microRNA-330-5p-mediated LDOC1

doi: 10.1186/s12935-019-1048-y

Figure Lengend Snippet: HAND2-AS1 is poorly expressed in cervical cancer and cells. a The heat map of some differentially expressed genes in the cervical cancer expression dataset GSE63678. The abscissa indicates the sample type and the ordinate indicates the gene expression level. The left dendrogram indicates the sample expression cluster, and the upper right histogram indicates color grade. b The expression of HAND2-AS1 in dataset GSE63678. The abscissa indicates the sample type, and the ordinate indicates the gene expression; the upper right indicates p value. c The expression of HAND2-AS1 in 68 cases of cervical cancer and adjacent tissues determined by RT-qPCR (N = 68), * p < 0.05 vs. the adjacent tissues. d The expression of HAND2-AS1 in human cervical cancer cell lines, * p < 0.05 vs. HUCEC cell line. All measurements were expressed by mean ± standard deviation. Paired t -test was used for comparison between cervical cancer tissues and adjacent tissues. The expression of each cell line was compared with one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated three times. HAND2-AS1 HAND2 antisense RNA 1, RT-qPCR reverse transcription quantitative polymerase chain reaction, ANOVA analysis of variance, HUCEC human cervical epithelial cell

Article Snippet: Normal human cervical epithelial cell lines (HUCEC) (BSC-00166804, ATCC, Manassas, VA, USA) were cultured in RPMI 1640 medium (12633012, Shanghai Haoran Bio Technologies Co., Ltd., Shanghai, China) containing 10% fetal bovine serum.

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Standard Deviation, Comparison, Reverse Transcription, Real-time Polymerase Chain Reaction